Nalkis Data Availability All relevant data are within the paper. The lateral-line system is well developed in adults of Lophiosilurusdistributed over the head and as a midlateral canal over the body from head to caudal-fin base. Morphometric variables were expressed as percentages of standard length, except for subunits of the head, expressed as percentages of head length. Caudal fin dorsally confluent with adipose fin; ventral finfold with reduced depth, but connected to anal and caudal fins.
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The mitochondrial genome was sequenced for the threatened Neotropical catfish L. Assembly into scaffolds using MIRA and MITObim software produced the whole, circularized mitochondrial genome, which comprises 16, bp and presents the typical gene arrangement of Teleostei mitochondria.
A phylogenomic analysis was performed after the concatenation of all proteins obtained from whole mitogenomes of 20 Siluriformes and two outgroups. The results confirmed the monophyly of nine families of catfishes and also clustered L. This is the first mitochondrial phylogenomics study for Pimelodoidea and the first mitogenome described for the Pseudopimelodidae family, representing an important resource for phylogeography, evolutionary biology, and conservation genetics studies in Neotropical fishes.
Keywords: Mitogenome, fish, next-generation-sequencing, Illumina, Pseudopimelodidae Catfishes Siluriformes are a highly diverse order composed of 39 families and more than 3, living species Eschmeyer and Fong, Threats such as overfishing and environmental degradation led to the inclusion of L.
Muscle fragments were obtained from a freshly captured L. Genomic DNA was extracted using a modified salting-out method Sunnucks and Hales, and nebulized for 6 min to obtain bp fragments.
Partial genome sequencing was carried out using a Nextera kit in a sixth of an Illumina HiSeq lane. Mitogenome coverage was obtained using Tablet software Milne et al. Mitos WebServer Bernt et al. Blast searches Altschul et al. The complete mitochondrial genome for L.
The assembly provided a circular mitogenome with Its gene content also followed the typical pattern for teleost mitogenomes Prosdocimi et al.
The phylogenetic relationship between L. The phylogenomic analysis produced a consistent tree in accordance with phylogenetic evidence obtained using rag1 and rag2 nuclear genes recovered using 3, base pairs Sullivan et al. The mitogenome described here is the first representative for the family Pseudopimelodidae.
Table 1 List of species, taxonomic information and accession numbers used in the phylogenetic analyses.
LOPHIOSILURUS ALEXANDRI PDF